PCNA Mouse Monoclonal Antibody （ PCNA小鼠单克隆抗体）

Attribute:

Mouse Monoclonal Antibody

Isotype:

IgG1

Purity:

Antigen Affinity Purification

Application:

ELISA, WB, IP,   IF

Calculated MW:

35kDa

Observed MW:

35kDa

Reactivity：

Human, mouse,   rat, Zebra fish

Immunogen：

Recombinant   Human PCNA protein expressed by  E.Coli   

Buffer：

PBS with 0.1% sodium azide and 50% glycerol,  pH 7.2

Storage:

Store at -20℃.   Do not aliquot

Recommended Dilution:

WB: 1:1000-2000

IP: 1:5000-10000

IF: 1:100-200

Background：

Proliferating   cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor   for DNA polymerase δ in eukaryotic cells and is essential for replication.   PCNA is a homotrimer and achieves its processivity by encircling the DNA,   where it acts as a scaffold to recruit proteins involved in DNA replication,   DNA repair, chromatin remodeling and epigenetics. PCNA gene is often stably   and constitutively expressed in most tissues and cells, it is commonly  used as a loading control for western blot   and other test.