Anti His-Tag Mouse mAb

抗体名：Anti His-Tag Mouse mAb
浓度：1mg/ml in PBS, pH 7.4, containing 0.1% proclin and 50% glycerol
应用范围：Western Blot;IP;IF
宿主：Mouse
适应物种：Wide Range
标记物：Purified
克隆性：单克隆
保存条件：Store at-20°C
形态：详询
亚型：Mouse IgG1
免疫原：A synthetic peptide HHHHHH coupled to KLH.
规格：100 ul
【描述】

Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells. Anti His-tag mouse mAb recognizes His-tag placed at N-terminus, C-terminus, and internal regions of fusion proteins.

【亚型】

Mouse IgG1

【目标蛋白】

A synthetic peptide HHHHHH coupled to KLH.

【成份】

1mg/ml in PBS, pH 7.4, containing 0.1% proclin and 50% glycerol

【贮存方法】

Store at-20°C

【推荐稀释度】

WB: 1:1000 ~ 1:10000,IP: 1:100 ~ 1:500,IF: 1:200 ~ 1:1000 Optimal dilutions should be determined by the end use

Fig: Western blot analysis of His-tag fusion protein with anti His-tag mouse mAb (5B8)diluted at 1: 2000(lane1) and 1: 5000(lane2).