Kappa light chain (rat)

Product number 161601D09H Antibody specificity Ig kappa chain C region (Kappa light chain) Species reactivity Rat Clone number KT102 Antibody isotype Mouse IgG1 Antibody form Intact antibody labelled with horseradish peroxidase Concentration 1 mg/ml in PBS containing 0.01% thimerosal as a preservative Cross-reactivity Not cross-react with rabbit, goat and human IgG, mouse IgG and IgM Applications Detection antibody for ELISA, Western blotting and antibody isotyping These are applications that have been tested. It does not mean that the antibody cannot be used for other applications 0.0 0.5 1.0 1.5 2.0 2.5 3.0 Rat IgG1 Rat IgG2a Rat IgG2b Rat IgG2c Rat IgM Mouse IgG Mouse IgM Rabbit IgG Goat IgG Human IgG OD405nm Figure 1. ELISA for cross-reactivity to various immunoglobulins Figure 2. Western blotting to rat immunoglobulins under reducing condition Suggested working concentrations ELISA: 1/2000 Western blotting: 1/500 – 1/10000 Optimal concentrations should be determined by the end user Preparation IgG is purified through a Protein A column and labelled with horseradish peroxidase Store 4 °C for 1 month, -20 °C or -80°C in aliquots. Avoid repeated freeze/thaw cycles Safety information This product contains thimerosal which is hazardous