6xHIS-Tag mouse mAb Cat#: 2088Synonyms:HHHHHHAttribute:Mouse Monoclonal Antibody Isotype:IgG1Purity:Protein A/G Affinity PurificationApplication:ELISA, WB, IPReactivity:Human,Mouse,Rat,Zebra fishImmunogen:Peptide HHHHHH Buffer:PBS with 0.1% sodium azide and 50% glycerol, pH 7.2Storage:Store at -20℃. Do not aliquotRecommended Dilution:WB: 1:2000-5000IP: 1:5000-10000 Background:The 6xHis tag, also known as polyhistidine tag, His6 tag and/or hexa histidine tag, is an amino acid motif consisting of at least 6 histidine residues fused to the carboxyl (C-) or amino (N-) terminus of a target protein in transfected cells. It is one of the simplest and most widely used purification tags in the world. 查看详情
FLAG-Tag mouse mAb Cat#: 2064Synonyms:DYKDDDDKAttribute:Mouse Monoclonal Antibody Isotype:IgG2aPurity:Antigen Affinity PurificationApplication:ELISA, WB, IP, IFCalculated MW:Observed MW:Reactivity:Human,Mouse,Rat,Zebra fishImmunogen:Peptide DYKDDDDK Buffer:PBS with 0.1% sodium azide and 50% glycerol, pH 7.2Storage:Store at -20℃. Do not aliquotRecommended Dilution:WB: 1:5000-10000IP: 3ug/sampleIF: 1:100-200Background:FLAG-tag is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. This antibody detects FLAG tag in most applications..查看详情